Fig. 3
a The mean corrected total cell fluorescences of bis-(1,3-dibutylbarbituric acid) trimethine oxonol (BOX) and propidium iodide (PI) over the course of the microfluidic desiccation process of untreated, trehalose-treated, and hypotonic + trehalose-treated blastospores. The graph is expanded to display the first 6 h of the experiment in which the hypotonic treatment (grey background area), the trehalose incubation (green background area), and the high-salt desiccation (orange background area) occur. The preceding cultivation in and subsequent rehydration to growth medium is indicated by the blue background. The full graph is shown in a miniature view to show the development beyond 6 h. All ctcfs were normalised to zero at t0. b Untreated and hypotonic + trehalose-treated blastospores at the end of the desiccation (t = 4.5 h). The images are compositions of the phase contrast, green, and red fluorescence channel images. The yellow and red ovals enclose points of interest, specified in the text