Fig. 2

Depicted is the fold change of GC–MS normalised peak areas of intracellular solutes to the untreated control (red dashed line) after, trehalose treatment, hypotonic treatment, or hypotonic + trehalose treatment. The red transparent area along the control line indicates the interquartile range concentrations of the untreated control. Lowercase letters indicate significant differences between the same solutes after the different treatments and to the control, but not to other solutes (n = 4, p < 0.05). Exact p-values, F-values, and employed tests for every solute are given in the Supplementary Materials 2